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L-Theanine vs. Melatonin: Which One Should You Take for Sleep?

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March 13, 2025

L-Theanine vs. Melatonin: Which One Should You Take for Sleep?

When it comes to improving sleep quality, both L-Theanine and melatonin are popular natural supplements. However, they work in different ways. Understanding their differences can help determine which one is right for you.

How L-Theanine Works

Dr. Michael Grandner, Sleep Expert, Professor of Neuroscience and Physiological Sciences:

“L-Theanine works by promoting calm brain waves, making it easier to wind down without sedation.”

L-Theanine is an amino acid found in tea leaves that promotes relaxation without causing drowsiness. It works by:

  • Increasing alpha brain waves, which encourage a calm state
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  • Enhancing GABA, serotonin, and dopamine production
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  • Reducing stress and anxiety

L-Theanine is best for those who struggle with stress-induced sleep disturbances (National Institutes of Health).

How L-Theanine Promotes Relaxation

L-Theanine is believed to enhance sleep by:

  • Boosting alpha brain waves, which promote relaxation
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  • Reducing stress and anxiety by regulating cortisol levels
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  • Enhancing the production of GABA, serotonin, and dopamine
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Studies suggest that L-Theanine can improve sleep efficiency and reduce the time it takes to fall asleep (National Institutes of Health).

How to Use L-Theanine for Sleep

L-Theanine is available in various forms, including:

  • Tea: Drinking green or black tea can provide a natural source of L-Theanine.
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  • Supplements: Capsules or tablets (100-400 mg) can be taken 30-60 minutes before bed.

Potential Side Effects

While generally safe, some individuals may experience:

  • Dizziness
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  • Gastrointestinal discomfort
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  • Lower blood pressure
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It's best to start with a lower dose and consult a healthcare provider if taking medications.

L-Theanine is a natural way to support relaxation and better sleep. By incorporating it into your nightly routine, you may experience improved sleep quality without the side effects of traditional sleep aids.

How L-Theanine and Caffeine Work Together

L-Theanine and caffeine are often taken together to enhance focus and reduce stress, but can this combination also benefit sleep quality? Understanding how these two compounds interact can help determine their effects on rest.

L-Theanine and caffeine have complementary effects:

  • L-Theanine: Promotes relaxation by increasing alpha brain waves and enhancing GABA production.
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  • Caffeine: Increases alertness by blocking adenosine, a neurotransmitter responsible for drowsiness.
  •    

When taken together, L-Theanine helps smooth out the stimulating effects of caffeine, reducing jitteriness and improving focus (National Institutes of Health).

Does the Combo Affect Sleep?

Dr. Areti Vassilopoulos, Sleep Expert, Pediatric Health Psychologist, Assistant Professor of Child Psychology:

“L-Theanine may counteract the jittery effects of caffeine—but timing matters. Avoid this combo too close to bedtime.”

While caffeine can disrupt sleep if consumed too late in the day, L-Theanine may help mitigate some of its negative effects by:

  • Reducing stress and promoting relaxation
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  • Lowering heart rate and blood pressure
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  • Enhancing overall sleep quality when taken separately from caffeine
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Best Practices for Taking L-Theanine and Caffeine

To optimize cognitive benefits without harming sleep, consider:

  • Taking the combination in the morning or early afternoon
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  • Using a 2:1 L-Theanine to caffeine ratio (e.g., 200 mg L-Theanine with 100 mg caffeine)
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  • Avoiding caffeine at least 6 hours before bedtime
  •    

While L-Theanine and caffeine together enhance focus and relaxation, caffeine should be avoided too close to bedtime. L-Theanine alone may still offer sleep benefits when taken separately.

When to Take L-Theanine for Sleep

To maximize sleep benefits, consider taking L-Theanine:

  • 30-60 minutes before bed: This allows enough time for the supplement to take effect.
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  • On an empty stomach: This may improve absorption and effectiveness.
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  • In combination with other sleep aids: L-Theanine pairs well with magnesium, melatonin, and valerian root.
  •    

Alternative Timing for Relaxation

L-Theanine is also effective during the day to promote relaxation without drowsiness. Some individuals take it:

  • In the morning to reduce daytime stress
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  • Before work or social events to ease anxiety
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  • Mid-afternoon to prevent an evening cortisol spike
  •    

For sleep support, L-Theanine is most effective when taken 30-60 minutes before bedtime. However, it can also be used during the day for relaxation and stress relief.

How Melatonin Works

Melatonin is a hormone naturally produced by the body to regulate sleep-wake cycles. Supplementing with melatonin can help by:

  • Signaling the body that it's time to sleep
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  • Resetting circadian rhythms for those with jet lag or shift work
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  • Improving sleep onset for individuals with insomnia
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Melatonin is ideal for those who have trouble falling asleep due to irregular schedules (National Institutes of Health). However melatonin also comes with certain side effects.

Which One Should You Take?

Dr. Suzanne Gorovoy, Sleep Expert, Clinical Psychologist, Behavioral Sleep Medicine Specialist:

“L-Theanine may reduce pre-sleep anxiety, while melatonin shifts circadian timing. They serve different purposes.”

If stress and anxiety keep you awake, L-Theanine may be the better option. If you have trouble falling asleep due to circadian rhythm disruptions, melatonin might be more effective.

Dr. Shiyan Yeo, Internal Medicine Physician, Sleep Medicine Expert:

“Both supplements can help—but they work best when paired with behavioral changes that support natural sleep.”

Both L-Theanine and melatonin offer unique sleep benefits. Choosing the right one depends on your specific sleep concerns and how your body responds to each supplement.

Take our Sleep Calculator and Chronotype Quiz to learn more about your sleep personality! If you're struggling with jet lag, try our Jet Lag Plan!

Dr. Shiyan Yeo

Dr. Shiyan Yeo is a medical doctor with over a decade of experience treating patients with chronic conditions. She graduated from the University of Manchester with a Bachelor of Medicine and Surgery (MBChB UK) and spent several years working at the National Health Service (NHS) in the United Kingdom, several Singapore government hospitals, and private functional medicine hospitals. Dr. Yeo specializes in root cause analysis, addressing hormonal, gut health, and lifestyle factors to treat chronic conditions. Drawing from her own experiences, she is dedicated to empowering others to optimize their health. She loves traveling, exploring nature, and spending quality time with family and friends.